Transfer RNA (tRNA) are types of RNA responsible for the transfer of specific amino acids to the growing end of a polypeptide chain during translation. R.Holly in 1965 gave clover leaf model of tRNA for yeast alanyl tRNA. It has four major sites - AA binding site, anticodon site, TYC loop and DHU loop. The chain is having unpaired base sequence CCA at 3′ end and G at 5′ end.