A DNA molecule has two unbranched complementary strands which are spirally coiled. The two chains are antiparallel, i.e., they run parallel but in opposite direction. One chain has the polarity 5′ → 3′ whereas, other has 3′ → 5′. Both are held together by hydrogen bonds between their bases, i.e., A = T and G ≡ C and the amount of adenine is equal to thymine and guanine equals to cytosine. The base ratio A + T / G + C may vary from one species to another but is constant for a given species. The purine and pyrimidines are always in equal amount (A + G = T + C) but A + T is not necessarily equal to G + C.