Q. Hardening in tissue culture is

A) Keeping $30{^\circ}$-$50℃$ temperature for about 30 minutes
B) Acclimatization of tissue culture plants slowly before growing in the field
C) Plunging the vials into water at $37{^\circ}$-$40℃$
D) None of the above
Explanation

Hardening is the acclimatization of plants formed by tissue culture before growing in the field to make them strong to adapt in new environment

Dr. Abubacker
Reviewed by Dr. Abubacker

Physics Educator & NEET/JEE Specialist

Ph.D. in Physics with 19+ years of teaching experience.