Q. Manipulation of DNA in genetic engineering became possible due to the discovery of
A) Restriction endonuclease
B) DNA ligase
C) Transcriptase
D) Primase
Explanation
Isolation of restriction endonucleases by Nathans and Smith (1970) made it possible to cut DNA at specific sites. Restriction enzyme can cut both strains of DNA when foreign nucleotides are introduced in the cell. They cleave DNA to generate a nick with a 5’ phosphoryl and 3’ hydroxyl terminus
Reviewed by Dr. Abubacker
Physics Educator & NEET/JEE Specialist
Ph.D. in Physics with 19+ years of teaching experience.