Q. In case of polymerase chain reaction, temperature, required for the steps A. Denaturation B. Annealing C. Extension
A) A-94$℃$, B-40$℃$, C-72$℃$
B) A-40$℃$, B-72$℃$, C-94$℃$
C) A-72$℃$, B-94$℃$, C-40$℃$
D) A-94$℃$, B-72$℃$, C-40$℃$
Explanation
A-Denaturation - $94℃$ B-Annealing - $40{^\circ} - 60℃$ C-Extension - $72℃$
Reviewed by Dr. Abubacker
Physics Educator & NEET/JEE Specialist
Ph.D. in Physics with 19+ years of teaching experience.