Q. Which of the following steps are catalyzed by $taq$ polymerase in a PCR reaction?
A) Denaturation of template DNA
B) Annealing of primers to template DNA
C) Extension of primer end on the template DNA
D) All of the above
Explanation
During extension, the enzymes Taq polymerase synthesizes the DNA segment between the primers. The two primers extend towards each other in order to copy the DNA segment typing between the two primers This step requires presence of deoxynucleoside triphosphate (d NTPs) and $Mg^{2 +}$ and occurs at $72℃$
Reviewed by Dr. Abubacker
Physics Educator & NEET/JEE Specialist
Ph.D. in Physics with 19+ years of teaching experience.