Q. Having become an expert on gel electrophoresis, you are asked to examine a gel for a colleague. Where would you find the smallest segment of DNA?
A) Near the positive electrode, farthest away from the wells
B) Near the negative electrode, close to the wells
C) Near the top, near the negative pole
D) Near the middle they tend to slow-down after the first few minutes
Explanation
A molecule of DNA can be cut into fragments by the enzyme restriction endonucleases. These fragments of DNA can be separated by a technique of gel electrophoresis. In this process the smallest segment of DNA travel towards anode (+ ve electrode), farthest away from the wells [image:11_image18.png]
Reviewed by Dr. Abubacker
Physics Educator & NEET/JEE Specialist
Ph.D. in Physics with 19+ years of teaching experience.