Q.
The figure shown three steps ($A,B,C$) of Polymerase Chain Reaction PCR. Select the option giving correct identification together with what represents?
A) B-denaturation at a temperature of about 98$℃$ separating the two DNA strands
B) A-denaturation at a temperature of about 50$℃$
C) C-extension in the presence of heat stable DNA polymerase
D) A-annealing with three sets of primers
Explanation
During extension, the enzyme DNA polymerase synthesizes the DNA segment between the primers. DNA polymerase is a heat stable enzyme
Reviewed by Dr. Abubacker
Physics Educator & NEET/JEE Specialist
Ph.D. in Physics with 19+ years of teaching experience.