A-plasmid, B-Boliver, C-Rodriquez. pBR322 vector was the first artificial cloning vector constructed in 1977 by Boliver and Rodriquez. It is widely used in gene cloning experiments in pBR322 p – Denotes that it is plasmid BR – stands for Boliver and Rodriquez who constructed this plasmid 322 is a number given to distinguish this plasmid from others developed in the same laboratory