Genetic engineering is a branch of biotechnology, which deals with the manipulation of genetic material by man. The technique of genetic engineering includes (i) formation of ‘recombinant DNA’ (ii) use of gene cloning (iii) gene transfer pBR 322 was the first artificial cloning vector constructed in 1977 by Boliver and Rodriguer. It is widely used in gene cloning experiments Restriction enzymes belongs to a class of enzymes called nucleases