Escherichia coli and Agrobacterium tumefaciens are the microbes found to be very useful in genetic engineering. E.coli is a motile, Gram negative, rod-shaped bacterium which is a normal inhabitant of human colon. It is most extensively used in bacterial genetic and molecular biology Agrobacterium tumefaciens is a soil bacterium. It has Ti-plasmid (tumour inducing plasmid) and it can be used for the transfer of a desired gene in dicot plants