Q. Which of the following steps are involved in the process of recombinant biotechnology? Arrange in correct order I. Extraction of the desired gene product II. Amplification of the gene of interest III. Isolation of a desired DNA fragment IV. Ligation of the DNA fragment into a vector V. Insertion of recombinant DNA into the host Correct order is
A) I, II, III, IV and V
B) III, II, IV, V and I
C) II, IV, V, III and I
D) I, IV, V, III and II
Explanation
Recombinant DNA technology involved the following steps (i) Isolation of DNA (ii) Fragmentation of DNA by restriction endonucleases (iii) Isolation of a desired DNA fragment (iv) Amplification of the gene of interest (v) Ligation of the DNA fragment into a vector (vi) Insertion of recombinant DNA into the host (vii) Culturing the host cells on a suitable medium at a large scale (viii) Extraction of the desired gene product (ix) Downstream processing of the products as finished product, ready for marketing
Reviewed by Dr. Abubacker
Physics Educator & NEET/JEE Specialist
Ph.D. in Physics with 19+ years of teaching experience.