Q. Which of the following statements are correct for the enzyme $taq$ polymerases? I. $Taq$ polymerase is thermally unstable II. It requires primers for carrying out the process of polymerization III. $Taq$ polymerase is isolated from thermophilic bacterium, $Thermus\ aquaticus$ Choose the correct option
A) I and II
B) I and III
C) II and III
D) I, II and III
Explanation
The final step in PCR is extension (polymerization), where in Taq DNA polymerase synthesizes the DNA region between the primers using deoxynucleotide triphosphates and $Mg^{2 +}$. It means the primers are extended towards each other so that the DNA segment lying between the two primer is copied. The optimum temperature for this polymerization step is $72℃$ Taq polymerase is thermostable enzyme, isolated from Thermophilic bacterium, Thermus aquaticus
Reviewed by Dr. Abubacker
Physics Educator & NEET/JEE Specialist
Ph.D. in Physics with 19+ years of teaching experience.