Q. PCR proceeds in three distinct steps governed by temperature. They are in order of
A) Denaturation, synthesis (polymerization), annealing
B) Annealing, synthesis (polymerization), denaturation
C) Synthesis (polymerization), annealing, denaturation
D) Denaturation, annealing, synthesis (polymerization)
Explanation
A single PCR amplification cycle involves three basic steps; denaturation, annealing and extension (polymerization) Denaturation – Melting of target DNA Annealing – Join Extension – Polymerisation
Reviewed by Dr. Abubacker
Physics Educator & NEET/JEE Specialist
Ph.D. in Physics with 19+ years of teaching experience.