Q. The role of DNA ligase in the construction of a recombinant DNA molecule is
A) Formation of phosphodiester bond between two DNA fragments
B) Formation of hydrogen bonds between sticky ends of DNA fragments
C) Ligation of all purine and pyrimidine bases
D) None of the above
Explanation
The role of DNA ligase in the construction of a recombinant DNA molecule is formation of phosphodiester bond between two DNA fragments. DNA ligase help in sealing gaps in DNA fragments Therefore, they act as a molecular glue. In 1969 Har Govind Khorana discovered DNA ligase in $T_{4}$-bacteriophage
Reviewed by Dr. Abubacker
Physics Educator & NEET/JEE Specialist
Ph.D. in Physics with 19+ years of teaching experience.