Q. PCR proceeds in three distinct steps governed by temperature, they are in order of
A) Denaturation, annealing, synthesis
B) Synthesis, annealing, denaturation
C) Annealing, synthesis, denaturation
D) Denaturation, synthesis, annealing
Explanation
Polymerase Chain Reaction (PCR) was developed by Kary Mullis. It is composed of denaturation of DNA at high temperature, annealing of primers at the ends of DNA strands and synthesis/polymerization by polymerase.
Reviewed by Dr. Abubacker
Physics Educator & NEET/JEE Specialist
Ph.D. in Physics with 19+ years of teaching experience.