Q. Which step was proved to be the main challenge in the production of human insulin by recombinant DNA technology?
A) Splitting A and B-peptide chain
B) Addition of C-peptide to proinsulin
C) Getting insulin assembled into mature form
D) Removal of C-peptide from active insulin
Explanation
The main challenge for the production of insulin using RiDNA technique was getting insulin assembled into a mature form. In 1983, Eli Lilly an American company, first prepared two DNA sequences corresponding to A and B chains of human insulin and introduced them into the plasmids of Escherichia coli to produce insulin chains. Chains A and B were produced separately, extracted and combined by creating disulphide bonds to form human insulin (humulin)
Reviewed by Dr. Abubacker
Physics Educator & NEET/JEE Specialist
Ph.D. in Physics with 19+ years of teaching experience.