Q. When a person with defective Adenosine Deaminase (ADA) was treated, which of the following steps were performing for gene therapy? I. Lymphocytes were extracted from the bone marrow of the patient II. Lymphocytes were grown in a culture out side the body III. Lymphocytes were transfected with the normal ADA genes IV. The transfected cells were returned to the patients
A) I, II and III
B) I, III and IV
C) II, III and IV
D) I, II, III and IV
Explanation
As a first step towards the gene therapy, lymphocytes a kind of white blood cells, are extracted from the bone marrow of the patient and are grown in a culture outside the body. A functional ADA, cDNA is then introduced into these lymphocytes, which the reinjected to the patients bone marrow but as these cells do not always remain alive, the patient requires periodic infusion or such genetically engineered lymphocytes
Reviewed by Dr. Abubacker
Physics Educator & NEET/JEE Specialist
Ph.D. in Physics with 19+ years of teaching experience.