Q. Sterilization of tissue culture medium is done by
A) Autoclaving of medium at $120{^\circ}$ for 15 min
B) Filtering the medium through fine sieve
C) Mixing the medium with antifungal agents
D) Keeping the medium at $- 20{^\circ}C$
Explanation
Autoclaving is commonly done at the temperature $121{^\circ}C$ for 15 min or at $134{^\circ}C$ for 3 min. The time required for sterilization depend upon the volume of medium in vessel
Reviewed by Dr. Abubacker
Physics Educator & NEET/JEE Specialist
Ph.D. in Physics with 19+ years of teaching experience.