(i) Collected gamete are made to form zygote in laboratory, in vitro fertilisation (IVF) (ii) Zygote or early embryo with up to 8 blastomeres is transferred into the oviduct (ZIFT) (iii) Zygote with more than 8 blastomeres is transferred into uterus (IUT) (iv) Fusion of gamete within the body of female (In vivo fertilisation) (v) Transfer of ovum from the donor to the oviduct of the recipient (GIFT) (vi) Sperms are injected into the ovum in vitro (ICSI)