Q. ELISA assay
A) Use complement mediated cells lysis
B) User a radiobelled second antibody
C) Involves addition of substrate which is converted into coloured end product
D) Requires red blood cells
Explanation
In ELISA (Enzyme Linked Immuno Sorbent Assay), the antibody is bound to an inert polymer support than exposed to the sample. The second antibody used is one that has an enzyme attached to it that converts a colourless or nonfluorescent substrate into a coloured or fluorescent produet.
Reviewed by Dr. Abubacker
Physics Educator & NEET/JEE Specialist
Ph.D. in Physics with 19+ years of teaching experience.