ELISA (Enzyme Linked Immuno Sorbent Assay), also known as an Enzyme Immuno Assay (EIA), is a biochemical technique used mainly in immunology to detect the presence of an antibody of an antigen in a sample. It is a useful tool for determining serum antibody concentrations (such as with the HIV test). The ELISA was the first screening test widely used for HIV because of its high sensitivity.