Q. Arrange the following steps in a correct sequence as per Gram’s staining technique I. Treatment with 0.5% iodine solution II. Washing with water III. Treatment with absolute alcohol/acetone IV. Staining with weak alkaline solution of crystal violet The correct sequence is
A) II$\rightarrow$I$\rightarrow$IV$\rightarrow$III
B) IV$\rightarrow$I$\rightarrow$II$\rightarrow$III
C) III$\rightarrow$II$\rightarrow$I$\rightarrow$IV
D) I$\rightarrow$IV$\rightarrow$III$\rightarrow$II
Explanation
Steps of Gram’s staining technique (i) Staining with weak alkaline solution of crystal violet (ii) Treatment with 0.5% iodine solution (iii) Washing with water (iv) Treatment with absolute alcohol/acetone
Reviewed by Dr. Abubacker
Physics Educator & NEET/JEE Specialist
Ph.D. in Physics with 19+ years of teaching experience.