During DNA replication, there occur a simultaneous continuous synthesis of DNA at both the strands of template in 5’ $\rightarrow$3’ direction of newly synthesised strand. Okazaki $et.\ al$, (1968) suggested that it is only one strand, which shows such a continuous replication (called leading strand), while other strand replicates in a discontinuous manner, $i.e$., synthesises short fragments called Okazaki fragments. This discontinuous strand is called lagging strand.