S-phase is the sub-phase between $G_{1}$-phase and $G_{2}$-phase, during which DNA synthesis or replication takes place. In animal cells, during the S-phase, DNA replication begins in the nucleus and the centriole duplication in the cytoplasm. The amount of DNA per cell doubles in the nucleus. If the initial amount of DNA is denoted as 2C, then it increases to 4C. However, there is no increase in the chromosome number