Limiting plasmolysis is the first stage of plasmolysis, during which the pressure on the wall is reduced and the wall contracts causing the reduction in cell wall simultaneously. During the second stage, the protoplast withdraws itself from the corners. This stage is known as incipient plasmolysis. Due to continued exosmosis, protoplasts shrink further and withdraws from the cell wall except on or few points. This is the last stage and is called evident plasmolysis