In a ringing or Girding experiment, the ring of bark, along with phloem is cut from the stem to represent the path of organic nutrients by phloem tissue. If phloem is not removed along with bark, supply of organic food will be continue and plant will survive. It xylem is girdled from main stem, supply of minerals and salts is stopped in the leaves and upper part of girdling site. So, wilting of leaves takes place after sometimes. In girdling experiment, root dies first as supply of food is stopped. In flowering plant, sieve tube transport food in the form of disaccaharides (sucrose)