Q. The following reaction represents $\alpha$-ketogulataric acid + $NH_{4}^{+} + NADPH\ \underset{dehydrogenose}{\overset{\ \ \ \ Glutamate\ \ \ \ \ }{\rightarrow}}$ Glutamate + $H_{2}O + NADP$
A) Reductive amination
B) Transamination
C) Amination
D) Nitrification
Explanation
The organic acid $–\alpha$-ketoglutaric acid, plays a key role in the synthesis of amino acid. The ammonia formed by nitrogen assimilation (i.e., reduction of nitrates), reacts with $\alpha$-ketoglutaric acid to form an amino acid, i.e., glutamic acid. In this process, $\alpha$-ketoglutaric acid comes from Krebs cycle and hydrogen is donated by the coenzyme NADH or NADPH. The reaction occurs in the presence of enzyme glutamic dehydrogenase
Reviewed by Dr. Abubacker
Physics Educator & NEET/JEE Specialist
Ph.D. in Physics with 19+ years of teaching experience.