The fixation of carbon dioxide in $C_{4}$-plants takes place in two places and by two different organic compounds. Phosphoenol pyruvate (PEP) is found in mesophyll cells, which primarily fixes atmospheric carbon dioxide into oxaloacetic acid (4C). RUBISCO is present in bundle sheath cells, where final fixation of carbon dioxide in hexose sugar takes place. Carbon dioxide is primarily fixed by PEP carboxylase because this enzyme has greater affinity to carbon dioxide than RUBISCO.