Carboxylation of one molecule of RuBP leads to the formation of 2 molecules of PGA $RuBP + CO_{2}\underset{carboxylase}{\overset{\ \ \ \ \ \ \ \ \ RuBP\ \ \ \ \ \ \ \ }{\rightarrow}}2 -$carboxyl 3-keto 1-5, bisphosphoribotol. 2-carboxyl 3-Keto 1-5-bisphosphoribotol $+ H_{2}O \rightarrow 2PGA$