$CO_{2}$ is required for photosynthesis is demonstrated by half-leaf experiment in which a part of a leaf is enclosed in a test tube containing some KOH soaked cotton (which absorbs $CO_{2}),\$while the other half is exposed to air. The setup is then placed in light for some time. On testing for starch later in the two halves of the leaf, the exposed part of the leaf tested positive for starch while the portion that was in the tube, tested negative. This showed that $CO_{2}$ is required for photosynthesis