The first reaction of photorespiration occurs in stroma of chloroplast. In this reaction, the RuBP (Ribulose 1, 5-biphophate) consumes one oxygen molecules in presence of enzyme RUBISCO. In peroxisome, the glycolate transferred from chloroplast, takes up oxygen and formed the glyoxylate whereas the $H_{2}O_{2}$ release as byproduct.