Q. Plasmid pBR322 has PstI restriction enzyme site within gene $amp^R$ that confers ampicillin resistance. If this enzyme is used for inserting a gene for $\beta$-galactoside production and the recombinant plasmid is inserted in an $E.\ coli$ strain
A) The transformed cells will have the ability to resist ampicillin as well as produce $\beta$-galactoside.
B) It will lead to lysis of host cell.
C) It will be able to produce a novel protein with dual ability.
D) It will not be able to confer ampicillin resistance to the host cell.
Explanation
[Solution] Inserting a gene at the PstI site inactivates $amp^R$ (insertional inactivation), so the recombinant cannot confer ampicillin resistance. Answer (4).
Reviewed by Dr. Abubacker
Physics Educator & NEET/JEE Specialist
Ph.D. in Physics with 19+ years of teaching experience.