During the process of gene amplification using PCR, if very high temperature is not maintained in the beginning, then which of the following steps of PCR will be affected first?

Question

Dr. Abubacker · Accepted Answer

[Solution] The first step, denaturation of dsDNA, needs a high temperature (about 94°C). Answer (2).

Q. During the process of gene amplification using PCR, if very high temperature is not maintained in the beginning, then which of the following steps of PCR will be affected first?

Explanation

Reviewed by Dr. Abubacker

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