Enolase works on 2-phosphoglyceric acid (3C-compound), Aconitase on citric acid (6C-compound). Fumerase on Fumaric acid (4C-compound) and alcohol dehydrogenase on acetaldehyde (2C-compound). Thus, increasing order of these enzymes based on the carbon number of the substrates on which they act is – IV, I, III, II.