In glycolytic pathway, 3PGAL is converted into 1, 3-diphosphoglyceric acid by an oxidation and phosphorylation reaction, which occurs in presence of H₃PO₄ and coenzyme NAD. 3-phosphoglyceraldehyde + NA$D^{+}$ + P$i^{- 2}$ $\rightarrow$ 3-phosphoglyceraldehyde dehydrogenase 1, 3-diphosphplyceric acid + NADH +$H^{+}$