Bohr’s effect A rise in $\rho CO_{2}$ or fall in pH decreases the oxygen affinity of haemoglobin, raising the $P_{50}$ value and shifts the curve to the right. This is called Bohr’s effect. Conversely, a fall in $\rho CO_{2}$ and rise in the pH increases oxygen affinity of haemoglobin. ($P_{50}$ value is the value of $\rho O_{2}$ at which haemoglobin is 50% saturated with oxygen to form haemoglobin