In the centre of each I-band is an elastic fibre called Z-line which bisects it. The thin filaments are firmly attached to the Z-line. The thick filaments in the A-band are also held together in the middle of this band by thin fibrous membrane called M-line. The A and I-band are arranged alternately throughout the length of myofibrils. The portion of the myofibrils between two successive Z-lines is considered as the functional unit of contraction called sarcomere