| In plant biotechnology, PEG is used in |
| a) | Protoplast isolation | b) | Cell culture preparation |
| c) | Protoplast fusion | d) | Hardening |
| (c)Somatic hybridization involves the fusion of protoplasts of two defferent species which resulted in hybrid. Naked protoplasts are obtained by dessolution of their cell walls by the mecerating enzymes such as pectinase and cellulas. Fusion of protoplasts from two defferent varieties can be enhanced by treating with polyethylene glycol (PEG) in presence of high voltage electric current. |